Protein Modification via Nitrile Oxide−Dehydroalanine Cycloaddition: Formation of Isoxazoline Ring on the Protein Backbone

Nitrile Oxide−Dehydroalanine Cycloaddition is a bioconjugation strategy between dehydroalanine-containing proteins and nitrile oxides, remarkably, in fully aqueous-buffered systems without an additional catalyst. This novel method of chemical protein modification forms isoxazoline ring enhancing the diversity function structure. The 1,3-dipolar cycloaddition reaction adds fluorescent label to for applications like live-cell imaging apoptosis detection. More information can be found Research Article by A. Phanumartwiwath et al.